Human group II 14 kDa phospholipase A2 activates human platelets

Group II phospholipase A2 in human gingiva with periodontal disease

Fourteen kilodalton phospholipase A(2) molecules (PLA(2)) are classified into two groups, I- and II-PLA(2), and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA(2) activity in gingival crevicular fluid (GCF) of patients with periodontal disease, without determining the group of the enzyme involved. In th...

Interaction of plant lipids with 14 kDa phospholipase A2 enzymes.

Several structurally related plant lipids were isolated and their effect was assessed on the enzyme activity of group I (pancreatic and Naja mocambique venom) and group II (Crotalus atrox venom) phospholipase A2 (PLA2) enzymes, with labelled Escherichia coli as an enzyme substrate. The neutral monogalactosyldiacylglycerol (MGDG) and negatively charged diacylglyceryl alpha-D-glucuronide (DGGA) d...

Phospholipase A2 Activates Hemostasis

BACKGROUND Phospholipases A(2) (PLA(2)) are aggressive enzymes that can destroy phospholipids of cell membranes. The resulting cell fragments trigger the kallikrein-mediated contact phase of coagulation. The aim of the present study was to expose citrated whole blood to PLA(2) and to quantify thrombin generation in recalcified plasma. METHODS Normal citrated blood was exposed to bovine pancre...

PLA2G2A (phospholipase A2, group IIA (platelets, synovial fluid))

Description Background: The phospholipase A2 (PLA2s) superfamily, consisting of at least 34 members subdivided into at least 15 groups, is a group of intracellular and secreted enzymes that hydrolyzes the sn-2 ester bond in glycerophospholipids found in lipoproteins and cellular membranes to liberate fatty acids and lysophospholipids that can act as intracellular second messengers or are furthe...

Human eosinophil group IID secretory phospholipase A2 causes surfactant dysfunction.